Potential of perillyl alcohol bearing PLGA microparticles in treatment of DMBA induced skin papilloma in Swiss albino mice

Farazuddin Mohammad, Ansari Mairaj Ahmed and Owais Mohammad

: J Bioengineer & Biomedical Sci

Abstract :

The present study is aimed to prepare and characterize a novel poly-lactic glycolic acid (PLGA) based microparticle formulation of perillyl alcohol (POH), and to evaluate its efficacy against treatment of di-methyl benzo anthracene (DMBA) induced skin papilloma in Swiss albino mice. Our data showed that POH bearing PLGA microparticles were of the size 445±153 nm with ζ-potential value of -7.56±0.88 mV. The entrapment efficiency of the active drug in particles was 58.4±3.5%. POH bearing PLGA microparticles were stable and released entrapped drug gradually over an extended time period. The ex-vivo efficacy of POH bearing PLGA microparticles was evaluated by assessing their ability to inhibit epidermoid carcinoma cell line (A253). The POH bearing microparticles when administered to tumor bearing animals caused greater tumor regression and increased survival rate (~80%) as compared to the group receiving free form of POH (survival rate 30%). The superiority of POH-PLGA microparticles over free form of POH was further evident from their ability to modulate apoptosis regulating factors.