Higher Institute of Biotechnology, Tunisia
Posters & Accepted Abstracts: J Pharmacogn Nat Prod
Polyphenolic contents and antioxidant and antimicrobial activities of Crataegus extracts from different vegetative and reproductive organs of Crataegus azarolus var. aronia harvested at different stages of growth have been determined. Three solvent systems with varying polarities (ethyl acetate, butanol and methanol) were used. The DPPH and ABTS+ assays were used to characterize the antioxidant actions of the extracts. Antimicrobial activity was determined by using disk diffusion and dilution assays for the determination of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values of each active extract. The highest amounts of polyphenols (8454.2l├?┬▒10.6 mg EAG/100g DW), flavonoids (2915.7├?┬▒20.9 mg RE/100g DW), and proanthocyanidins (3923.6├?┬▒227 mg cyanidin chloride/100g DW) were shown by the polar sub-fraction methanolic extracts. All extracts show a good antioxidant and antimicrobial activities, the most efficient being fresh young leaves, fresh floral buds and fresh green fruits. The activities seem to be especially bound to the total phenolic, proanthocyanidin and flavonoid contents.
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