PCR based analysis for the detection of Xanthomonas vesicatoria causing pepper and tomato bacterial spot

Annual Conference on Bioscience

September 12-13, 2016 Berlin, Germany

Nazila Farhangzad, Solmaz Mosayyebi and Afife Izb�±rak

Hacettepe University, Turkey

Posters & Accepted Abstracts: J Bioeng Biomed Sci

Abstract :

Xanthomonas vesicatoria is a major pathogen of tomato (Solanum lycopersicum) and pepper (Capsicum spp.). Bacterial spot caused by Xanthomonas vesicatoria is one of the most important diseases in many countries throughout the world. The disease causes considerable crop loses specially in areas of warmer and humid climate. In this study, 50 pathogenic Xanthomonas strains were isolated from tomatoes and peppers with bacterial spot in Turkey and USA. In continuously, 29 samples of 50 Xanthomonas strains were determined as Xanthomonas vesicatoria in the presence of 517 bp fragment of rhs gene by using specific XCVF and XCVR primers. We investigated that this protocol can be used as a reliable diagnostic tool for specific detection of Xanthomonas vesicatoria in tomato and pepper.

Biography :

Email: nfarhangzad@yahoo.com