Wananit Wimuttisuk
National Center for Genetic Engineering and Biotechnology, Thailand
Posters & Accepted Abstracts: Metabolomics
Despite decades of research, the cause of delayed ovarian development in domesticated black tiger shrimp Penaeus monodon remained poorly understood. Thus, unilateral eyestalk ablation was routinely performed on the broodstock to force ovarian development. To circumvent this, we focused on the optimization of lipid content in shrimp feed as addition of C20:4, C20:5 and C22:6 in food pellets led to improved ovary maturation, fecundity and oogenesis in other crustaceans. Here we compared the effects of two shrimp feed: food pellets for broodstock and a live feed called polychetes Perineresis nuntia on shrimp ovarian maturation, gene expressions, and the lipid profiles in shrimp ovaries and hepatopancreases. Gas chromatography analysis revealed that food pellets contained more total fat, C20:5, and C22:6 than polychetes. Nevertheless, shrimp fed with polychetes still have more developed ovaries. Subsequently, HPLC analyses and enzyme immunoassays indicated that the amounts of prostaglandin E2 (PGE2) and prostaglandin F2�± (PGF2�±), the two C20:4-derived eicosanoids, were significantly higher in the hepatopancreas of shrimp fed with polychetes. Microarray analyses also revealed significant changes in gene expression of the eicosanoid biosynthesis pathway in both ovaries and hepatopancreases over the course of ovarian maturation. Based on these data, we believe that eicosanoids are the determining factors that regulate reproductive maturation in P. monodon. By understanding the dietary lipid requirements and gene regulations in female broodstock, we may be able to optimize the shrimp feed for a more sustainable shrimp larvae production in the future.
Email: wananit.wim@biotec.or.th
Metabolomics:Open Access received 895 citations as per Google Scholar report
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