Molecular diversity of root nodule bacteria from different leguminous crop

Annual Conference on Bioscience

September 12-13, 2016 Berlin, Germany

Pooja Agrawal

Madhya Pradesh Council of Science and Technology, India

Posters & Accepted Abstracts: J Bioeng Biomed Sci

Abstract :

A total of 210 samples of four legumes i.e., 85 of Cicer arietinum, 74 of Glycine max, 21 of Vigna radiata and 40 of Cajanus cajan were collected from different fields of District Sagar and were processed for the isolation of root nodule bacteria, Rhizobium. A total of 247 bacteria were identified as Rhizobium, out of which 94 from Cicer arietinum, 69 from Glycine max, 43 from Vigna radiata and 41 from Cajanus cajan, on the basis of Lactose agar test, Nitrate reduction test, citrate utilization test, motility, oxidase, catalase different staining technique i.e., Gram�s staining and carbol fuschin staining. All the isolates were tested for phosphatase activity on solid plate assay. Maximum phosphatase production was noted in the test strains were selected for siderophore and indole acetic acid production. Ten selected rhizobia were subjected to RAPD and ARDRA analysis to identify the species. On the basis of RAPD and ARDAR all the 10 isolates were identified as Rhizobium meliloti. Higher phosphatase activity was noted in ten Rhizobium isolates i.e., Rhizobium S81, Rhizobium G04, Rhizobium G16, Rhizobium G20, Rhizobium G77, Rhizobium S43, Rhizobium S81, Rhizobium M07, Rhizobium M37, Rhizobium A15, Rhizobium and Rhizobium A55.

Biography :

Email: dr.poojagrawal@gmail.com