Metabolic reprogramming of macrophages exposed to Pseudomonas aeruginosa biofilms

13^th International Conference on Metabolomics and Systems Biology

October 11-12, 2018 | Zurich, Switzerland

Valérie Copie, Amanda L Fuchs and Mary Cloud B Ammons

Montana State University, Bozeman, USA
Idaho Veterans Research & Education Foundation, USA

Posters & Accepted Abstracts: Metabolomics (Los Angel)

Abstract :

Currently, the annual economic cost of chronic wounds exceeds $1 billion in the United States, and the incidence of nonhealing human wounds is expected to dramatically increase in the next several years due to emerging Type 2 diabetes epidemics. Several common characteristics typical of chronic wounds include tissue colonization by persistent antibioticresistant pathogenic microbial biofilms, excessive inflammation, and failure of human cells to resolve the wound. Pseudomonas aeruginosa is one of the predominant opportunistic pathogens that colonizes greater than 50% of all chronic wounds in the US and is a serious health threat. To better understand the molecular processes by which P. aeruginosa biofilms interfere with human macrophage immune responses, we have undertaken nuclear magnetic resonance (NMR)-based metabolomics studies of activated and resting macrophages. The studies aim to probe the metabolic reprogramming of these immune cells as result of exposure to secreted molecules produced by P. aeruginosa biofilms, using an in vitro host-pathogen co-culture model. Herein, we present our recent NMR-based metabolomics results demonstrating the presence of a significant metabolic shift between different macrophage phenotypes. This metabolic phenotyping is correlated with fluorescence-activated cell sorting (FACS) analysis which has been employed to characterize the relationship between metabolic profiles and macrophage immunomodulation (i.e. macrophage polarization into pro- or anti-inflammatory M1 or M2 subpopulations, respectively), resulting from macrophage exposure to secreted molecules from co-culture P. aeruginosa biofilms.

Biography :

E-mail: vcopie@montana.edu