Metabolic reconstruction of selected functions in computational annotation of hypothetical proteins from Microcystis aeruginosa NIES-843

2^nd International Conference and Exhibition on Metabolomics & Systems Biology

April 08-10, 2013 Hilton Chicago/Northbrook, USA

Paramasivan Manivannanand and Gangatharan Muralitharan

Accepted Abstracts: Metabolomics

Abstract :

Bloom forming Microcystis aeruginosa is known to have high plasticity of genome due to lateral gene transfer. The cyanobacteria has putative gene clusters for NRPS/PKS system for secondary metabolites production in eliciting antimicrobial and allelopathic substances. Recently microvirin, a lectin derived from the organism has shown anti-HIV activity. Furthermore, hypothetical proteins are those proteins that confer a function without comprehensive or curated annotations. In view of the above, the present work is aimed at deciphering functions for 187 hypothetical proteins from Microcystis aeruginosa NIES-843. The in silico methodology adopted for function predictions include clustering and regulon analysis for characterizing functions that encompass selected functions based on metabolomic assessment. The results affirm that the computational assessment of hypothetical proteins is categorized among functions like synteny, restriction-modification system, methionine salvage pathway and hydrogen uptake system. The present work would enable experimental validation of functions for hypothetical proteins for cyanobacterial genomes. The work flow could be rationalized for functional annotation of hypothetical proteins for all bacterial hypothetical proteins.