Lactococcus lactis, an attractive bacterium for expression and functional characterization of membrane proteins

7^th International Conference on PHARMACEUTICS & ADVANCED DRUG DELIVERY SYSTEMS

March 27-28, 2023 | London, UK

Frelet-Barrand Annie

FEMTO-ST Institute, France

Posters & Accepted Abstracts: J Med Chem

Abstract :

Membrane proteins play key roles in most crucial cellular processes, ranging from cell-to-cell communication to signaling processes. Despite recent improvements, the expression of functionally folded membrane proteins in sufficient amounts for functional and structural characterization remains a challenge. Indeed, it is still difficult to predict whether a protein can be overproduced in a functional state in some expression system(s), though studies of high-throughput screens have been published in recent years. Prokaryotic expression systems present several advantages over eukaryotic ones. Among them, Lactococcus lactis (L. lactis) has emerged in the last two decades as a good alternative expression system to E. coli. The purpose here is to describe L. lactis and its tightly inducible system, NICE, for the effective expression and functional characterization of membrane proteins from both prokaryotic and eukaryotic origins.