iTRAQ-based analysis of complex serum proteome alterations in Indian zebu cattle subjected to heat stress

7^th International Conference and Expo on Metabolomics

November 14-16, 2016 Orlando, Florida, USA

Khan Farheen Badrealam, Sohan Vir Singh and Ashok Kumar Mohanty

National Dairy Research Institute, India

Posters & Accepted Abstracts: Metabolomics

Abstract :

Heat stress is a major stressor which affects animal�s performance substantially and molecular basis coherent with the ability of Bos Indicus to survive and adapt to heat stress. Reckoning with these, the present study was planned to examine the complex serum proteome alterations in Indian zebu cattle following heat stress and normothermia applying isobaric Tags for Relative and Absolute Quantification (iTRAQ) proteomics approaches. The data of the present study suggests that out of the total 144 proteins identified, 53 proteins were differentially expressed after 4 h of heat stress (23 proteins up-regulated and 30 proteins down-regulated), whereas 48 (23 proteins up-regulated whereas 25 down-regulated) and 51 (18 proteins upregulated whereas 33 down-regulated) proteins were differentially expressed after 24 and 48 h of recovery period respectively. Intriguingly, this is the seminal report investigating the differential proteome profile of Sahiwal serum samples in response to heat stress. The identified proteins were subjected to analysis for their prospective involvement in various biological, cellular and molecular processes including thermoregulatory mechanism. The high confidence dataset generated herein may plausibly foster identification of prospective biomarkers related to heat stress in Indian zebu cattle; nonetheless, the findings of the present study significantly advances our understanding of the physiological aspects of heat stress induced responses in Sahiwal cattle.

Biography :

Email: farheenkbiot@gmail.com