Investigation of mRNA expression of photosystem II D1 protein (psbA) and usnic acid production in manganese tolerance in lichen Xanthoria parietina

2^nd International Summit on Integrative Biology

August 04-05, 2014 Hilton-Chicago/Northbrook, Chicago, USA

Seyma Akarsu, Hulya Silah and Dilek Ozakca

Accepted Abstracts: J Comput Sci Syst Biol

Abstract :

In this study, the effects of short-term manganese tolerance on the lichen Xanthoria parietina were investigated at the physiological and transcriptional levels. Usnic acid production was increased with manganese application depending on concentration and application period. The expression of the photosystem II D1 protein ( psbA ) gene and non-reducing polyketide synthases ( PKS ) gene was quantified using semi-quantitative RT-PCR. Increased non-reducing PKS and psbA mRNA transcript levels were observed in the X. parietina thalli that were treated with different concentrations of manganese. The results showed that there was positive correlation between usnic acid production and non-reducing PKS transcript levels. In the present study, the data also demonstrated that non-reducing PKS and psbA mRNA transcript levels could be play important roles in manganese tolerance.