Fadipe V O1, Mongalo N I2 and Opoku A R1
1University of Zululand, South Africa 2University of South Africa, South Africa
Posters-Accepted Abstracts: Med chem
Curtisia dentata is traditionally used in African traditional medicine to treat and manage sexually transmitted infections, diarrhoea, stomach complaints and as a purgative. C. dentata leaves were collected from Buffelskloof Private Nature Reserve in Mpumalanga province, South Africa. The ethanol, chloroform, ethyl acetate and acetone extracts were evaluated for antimicrobial activity using micro dilution assay against ATCC strains of Escherichia coli, Pseudomonas aeruginosa, Mycobacterium smegmatis, Mycoplasma hominis, Candida albicans and some clinical isolates of Moraxella catarrhalis, Proteus mirabilis and Staphylococcus aureus isolated from HIV patient. Acetone extract exhibited lowest MIC of 0.01 mg/ml against Candida albicans compared to other extracts. Besides lupeol, betulinic acid and ursolic acid, �²-sitosterol was isolated for the first time from C. dentata leaves and exhibited moderate antimicrobial activity with MIC values ranging from 0.20 to 6.25 mg/ml. Furthermore, the ethanol extract and the four isolated compounds revealed MIC index of less than 4 suggesting that their effect is bactericidal. The ethanol extract revealed the best total activity of 2400 ml/g against Mycoplasma hominis compared to other extracts. Lupeol exhibited low selectivity index indicating that the compound had the similar cytotoxicity and antimicrobial activity. The cytotoxicity of the isolated compounds was further investigated against the Human Embryonic Kidney (HEK293) and Human Hepatocellular Carcinoma (HepG2) cell lines using the MTT assay. Ursolic acid exhibited the lowest LD50 of 122.4 �¼g/ml against HEK293 cell line, while lupeol exhibited LD50 of 278.8 and 289.4 �¼g/ ml against HEK293 and HepG2, respectively. The ethyl acetate and acetone extracts were further investigated for antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH). The acetone extract exhibited 53.6% inhibition against DPPH at a concentration of 1 mg/100 ml. The findings of the current work validate the use of the plant species in the treatment of various human infections. The biological activity of the extracts, particularly the ethanol extract, may possibly be attributed to the isolated compounds. There is a need to explore the biological activity of the derivatives of the compounds isolated against other human pathogenic strains.
Email: vobfadipe@yahoo.com
Medicinal Chemistry received 6627 citations as per Google Scholar report
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