Z L Mkhize Kwitshana
Mangosuthu University of Technology, South Africa
Posters & Accepted Abstracts: J Bioengineer & Biomedical Sci
High-risk human papillomavirus (hr-HPV) infections and low-grade squamous intraepithelial lesions occur frequently in young women. The available vaccines cater for up to nine hr-HPV genotypes, which may not necessarily be the most predominant types in every region worldwide. The objective of this study was to describe the hr-HPV genotypes present among HIV uninfected and infected young women in rural areas of the KwaZulu-Natal province of South Africa. Cervico-vaginal lavages were obtained from sexually active young women recruited from high schools in KwaZulu-Natal (n=1223). HPV detection and genotyping were done by the polymerase chain reaction using GP5+/GP6+ primers and enzyme immunoassay. HIV testing was done on serum using rapid tests. Of the 1223 cervico-vaginal lavages, 301 (25%) were positive for hr-HPV. The HPV prevalence was higher in HIV infected (32.2%, 95% CI, 0.27-0.38) than in HIV uninfected women (22.5%%, 95% CI, 0.21-0.26), p=0.001. Similarly, multiple infections were slightly more common in HIV infected (59.3%) than in HIV uninfected women (53.5%), p=0.37. The nine most predominant genotypes in descending order were HPV types 16 (n=99, 22.1%), type 51 (n=58, 12.9%), type 18 (n=56, 12.5%), type 35 (n=50, 11.1%), type 33 (n=47, 10.8%), type 56 (n=42, 9.3%), type 45 (n=34, 7.6%), type 52 (n=32, 7.1%) and type 59 (n=31, 6.9%). Specifically, HPV 35, 51, 56 and 59 (40.6%) were among the most prevalent in the schools of KwaZulu-Natal not covered by the ninevalent vaccine.