Evidence of phenylalanine pathway in resveratrol-producing Alternaria sp.: Substrate flow and enzyme activities

Metabolomics:Open Access

ISSN: 2153-0769

Open Access

Evidence of phenylalanine pathway in resveratrol-producing Alternaria sp.: Substrate flow and enzyme activities

3rd International Conference and Exhibition on Metabolomics & Systems Biology

March 24-26, 2014 Hilton San Antonio Airport, San Antonio, USA

Junling Shi

Accepted Abstracts: Metabolomics

Abstract :

Alternaria sp. MG1 is an endophytic fungus isolated from grape and has capability in producing resveratrol, indicating the occurrence of resveratrol biosynthesis pathway in this strain. Different substrates and C 13 -glucose were used in the culture of Alternaria sp. MG1 resting cells. The intermediates accumulated during the culture were identified using high performance liquid chromatography (HPLC) and mass spectrograph. During the biotransformation, activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans-cinnamate 4-hydroxylase (C4H) and 4-coumarate-CoA ligase (4CL) were analyzed and tracked. The reaction system contained 100 mL 0.2 mol/L phosphate buffer (pH 6.5), 120 g/L Alternaria sp. MG1 cells, 0.1 g/L MgSO 4 and 0.2g/L CaSO 4 and different substrates according to the experimental design. The biotransformation was carried out for 21 hours at 28C and 120 rpm. Resveratrol formation was identified when phenylalanine, tyrosine, cinnamic acid and p-coumaric acid were separately used as only substrate. Accumulation of cinnamic acid, p-coumaric acid and resveratrol and activities of PAL, C4H and 4CL were identified and changed in different trends during transformation with phenylalanine as only substrate. C 13 isotope analysis showed 1 or 7 carbon atoms were transferred from glucose to the intermediates and substrates in phenylalanine pathway, indicating the occurrence of this pathway in Alternaria sp.

Biography :

Junling Shi completed her Ph.D. from Northwest Agriculture and Forestry University and postdoctoral studies from Jiangnan University. She is a Professor of Northwestern Polytechnical University now. She has published more than 30 papers in reputed journals. Her major research field is endophytic fungi and fungal metabolism.

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