Epstein-Barr virus infection in a group of post renal transplant patients in two main renal transplant centers in a South Asian country

17^th International Conference on Nephrology & Urology

March 12-13, 2018 London, UK

A I K Mahanama and J I Abenayake

Medical Research Institute, Sri Lanka

Posters & Accepted Abstracts: J Nephrol Ther

Abstract :

Pre-transplant serology guided monitoring of high-risk renal transplant recipients {donor positive (D+)/ recipient negative(R-)} is recommended to initiate early pre-emptive therapy to prevent EBV disease/post-transplant lymphoproliferative disorder. Sri Lanka is yet to implement this due to limited resources, although many renal transplants are performed annually. This descriptive cross-sectional study aims to evaluate the practice of pre-transplant EBV serology testing, determine the magnitude of high-risk population and to describe active EBV infection within first post-transplant year. Plasma of 118 adult posttransplant patients over four months tested for EBV DNA with a commercially validated quantitative real-time PCR kit. EBV serology and other transplant details collected using clinical records. Majority were males with mean age of 44.97 years (SD 12.48). 54.3% were >6 months post-transplant, 90.8% received a live related kidney all were on maintenance with Tacrolimus, MMF and Prednisolone. Pre-transplant serology is available in 37/118 recipients. Donor serology available in 27/118 recipients, 12 IgM, 13 IgG and two had both. Sero-compatibility details available in 15/118 with 20% being D+/R- . All samples were negative for EBV DNA despite ensuring maximum viral DNA recovery. Study concludes that pre-transplant screening for EBV infection was poor with incorrect antibody selection in donor. Many belonged to high-risk category emphasizing the need for implementing routine pre-transplant EBV screening guided post-transplant monitoring to improve the quality of care. None had active EBV infection, use of single sample per patient, few recruits were in early transplant period and short study period may have affected the EBV DNA detection rate.

Biography :

A I K Mahanama has completed her MBBS degree from the Faculty of Medicine, Colombo and Post Graduate Diploma in Medical Microbiology from the Post Graduate Institute of Medicine, Sri Lanka. She currently works as a Postgraduate trainee in MD in Medical Virology at Medical Research Institute, a premier research institution in Sri Lanka. She has several local and international publications in her name and is also engaged in several research studies at the moment.
Email:adhyanaindunil@gmail.com