Ehsan Sadeghi and Ali Reza Fakhari
Shahid Beheshti University, Iran
Posters & Accepted Abstracts: Med chem (Los Angeles)
Electromembrane extraction (EME) is a sample preparation technique in pharmaceutical, chemical, clinical and environmental analysis. This technique uses electro-migration across artificial liquid membranes for selective extraction of analytes and sample enrichment from complex matrices. This method has many advantages such as simplicity, rapid, low-cost, low LOD, high pre-concentration factor and high recovery. In the present work, simultaneous pre-concentration and determination of two basic drugs namely metoclopramide (MCP) and ondansetron (OSN) were studied using EME as a suitable extraction method, followed with capillary electrophoresis (CE) using ultraviolet (UV) detection as separation technique. The drugs were extracted from 4 ml sample solutions, through a supported liquid membrane (SLM) consisting 2-nitrophenyloctylether (NPOE) impregnated in the walls of a polypropylene hollow fiber, and into a 20 mL acidic aqueous acceptor solution resent inside the lumen of the hollow fiber with a potential difference applied over the SLM. The variables of interest, such as chemical composition of the organic liquid membrane, stirring speed, extraction time and voltage, pH of donor and acceptor phases and salt effect in the EME process were investigated and optimized. Under optimal conditions NPOE as SLM, stirring rate of 1000 rpm, 200 V potential differences, 20 min as the extraction time, acceptor phase HCl (pH 1.0) and donor phase HCl (pH 1.5). After the microextraction process, the extracts were analyzed by CE with optimum conditions phosphate running buffer (pH 2.0), applied voltage of 20 kV and 25├?┬░C. Under the optimum conditions, limits of detection (LOD) and quantification (LOQ) for MCP and OSN were 2.31-2.68 and 7.72-8.91 ng mL-1 respectively. Pre-concentration factor and RSD for five replicates of each drug were calculated to be 200 and 4.06-3.93 respectively. Finally, the applicability of this method was studied by the extraction and determination of these drugs in urine samples with recovery percentages of 87├ó┬?┬?92%.