Effect of 4-phenylbutyrate on palmitate induced oxidative stress and renal injury

6^th Annual conference on Clinical & Pediatric Nephrology

May 09-10, 2016 New Orleans, USA

Heba Al-khaznadar, Myriam Jaam and Shankar Manusamy

Qatar University, Qatar

Posters & Accepted Abstracts: J Nephrol Ther

Abstract :

Obesity is a significant risk factor for chronic kidney disease. Obesity is shown to increase oxidative stress in the kidney and cause renal injury. Previous studies suggest that 4-phenyl butyric acid (4-PBA) has a protective role against saturated fatty acid induced renal cell death. The primary goal of this project is to evaluate the effect of 4-PBA against palmitate induced oxidative stress and renal injury. NRK-52E cells (a rat renal proximal tubular cell line) were grown to 70% confluency and divided into four treatment groups; control, 250 �¼M palmitic acid (PA), 5 mM 4-PBA and the combination of 250 �¼M PA and 5 mM 4-PBA. After 24 hours treatment period, cells were assessed for oxidative stress by dichlorofluorescein (DCF) staining and for changes in cell viability using the MTT assay. Results had shown that PA treatment markedly increased the DCF fluorescence (3.089�±1.87 fold increase over control) and reduced cell viability (83�±6% compared to control) in NRK cells. On the other hand, the combination group showed no alterations in DCF fluorescence (1.038�±0.26 fold change over control) and cell viability (96�±2% compared to control). In conclusion, findings of this research suggest that 4-PBA protects NRK cells against PA induced oxidative stress, which was demonstrated by the low DCF fluorescence in the combination group compared to the PA treated group. In addition, 4-PBA also protects NRK cells against PA induced cell death.

Biography :

Email: ha092143@student.qu.edu.qa