Bojnord University School of Medicine, Iran
Posters & Accepted Abstracts: J Nephrol Ther
Introduction & Aim: Nicotine is the most chemical agent in the cigarette that has toxic effects on tissue cells and adhesion molecules that associated with the extracellular matrix. The aim of this study was to assess the effects of maternal nicotine exposure during pregnancy and lactation periods on fibronectine expression in offspring kidney extracellular matrix. Materials & Methods: The 24 female pregnant Balb/c mice were randomly divided into four groups as follow: E1 group received only 3mg/kg/day nicotine intraperitonealy (IP) from the 6th day of uterine life to end of pregnancy. E2 group received same dose of nicotine in similar way from the first day of delivery to the end of lactation. C1group received 3ml/kg/day normal saline parallel to the A1 group and the C2 group received equal volume of normal saline parallel to the A2 group. In the first and twentieth days after delivery offspring kidneys were extracted for Immunohistochemical (IHC) and Real-Time Polymerase Chain Reaction (RT-PCR) studies. Results: Offspring kidney IHC sections in E1 group showed fibronectine reaction was significantly increased in the glomerule of new born (p<0.05). Also the internal diameter of convoluted tubule reduced in E1 group. RT-PCR results demonstrated that the mRNA level of the fibronectin in E2 group was up regulated significantly compared to the other group. Conclusions: Maternal nicotine administration not altered fibronectine expression in gestation life.
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