Copper( II ) Complex-Based fluorescence sensors for nitric oxide detecting and imaging

International Conference and Exhibition on Biosensors & Bioelectronics

May 14-16, 2012 Embassy Suites Las Vegas, USA

Chunying Duan, Xiaoyue Hu, Xiaolin Zhang, Cheng He, Yongming Bao and Qin Tang

Scientific Tracks Abstracts: J Biosens Bioelectron

Abstract :

Nitric oxide (NO) has received great attention since the discovery that it is an important signaling agent in biological systems. The method to directly detect NO in real time and in vivo is critical to the investigation of the multiple biological roles of NO. While a variety of quantification techniques developed, fluorometric techniques become the gold standard for NO sensing, owing to their sensitivity and high spatiotemoral resolution, when combined with microscopy.The first generation of NO fluorescence sensors was based on organic molecules, which has a critical limitation that it cannot provide direct and realtime detection of NO. To resolve this problem, a novel strategy involving the reaction of transition metal and NO was explored recently. It was inspired by the interaction of NO and metal centers of enzyme in organism. Especially, the recent research showed that the sensors based on the Cu(II)-complex were more effective. According to above, we designed and synthesized a series of new Cu(II)-complex luminescence sensors for detection of NO, incorporating rhodamine/naphthalimide moiety as the luminescence active unit and polyamine as the efficient Cu(II) chelator. These sensors could be regarded as an approximate model of the enzyme center, which underwent a redox reaction with NO to restore the emission of fluorophore, giving an enhancement response. The new sensors exhibited high sensitivity (detection limit 1nM) and excellent specificity toward NO, and have been applied to NO imaging successfully.

Biography :

Chunying Duan was born in 1967, China. He completed his Ph.D. at Nanjing University in 1992. Then he started his academic career in the Department of Chemistry at Nanjing University. Since 2006, he has worked at Dalian University of Thechnology as the deputy director of State Key Laboratory of Fine Chemicals. His research interests cover aspects of supramolecular chemistry, coordination chemistry, molecular sensors, and chiral materials.