Antioxidant activity and hepatoprotective effects of Centaurea incana on CCL4-induced liver toxicity in rats

7^th European Biosimilars Congress

May 15-16, 2017 Munich, Germany

Boubellouta Houria, Khelifi Touhami Fatima, Kermandji Mohamed Azed and Bellatrache Cherifa

University of Bejaia, Algeria
Constantine 1 University, Algeria

Posters & Accepted Abstracts: J Bioanal Biomed

Abstract :

Aim: The aim of the present study was to investigate the potential antioxidant and hepatoprotective effects of Centaurea incana on the free radical damage of liver caused by carbon tetrachloride in rats. Methods: For the study of preventive effect of methanolic extract of Centaurea incana on CCL4-induced hepatotoxicity, our study was conducted on rats as follows: The animals were randomly divided into 4 different groups comprising of 7 animals each. Group I served as control and received an injection of vehicle (olive oil) alone; Acute liver injury in rats was induced by a single intra-peritoneal injection with CCl4 dissolved in an equal volume of olive oil at a dose of 3 mL/kg body weight, group II, which is well documented to induce hepatotoxicity. Group III was administered methanolic extract of Centaurea incana at a dose of 500 mg/kg alone. In group IV, methanolic extract of Centaurea incana was administered at a dose of 500 mg/kg and was injected with CCl4 i.p. at a dose of 3 mL/kg body weight. After 4 weeks of treatment, all of the animals were sacrificed 24 h after administration of CCl4, and blood was collected, serum separated and stored at â��20�ºC. Results: The single intra-peritoneal injection with CCl4 caused severe hepatotoxicity in rats, as evidenced by the significant elevation of serum AST and ALT activities after the administration of CCl4. the concentration of MDA, an end product of lipid peroxidation, in the rats treated with CCl4 was increased 2.7-fold when compared with the vehicle control rats. However, pre-treatment with Centaurea incana significantly prevented the elevation of serum AST and ALT activities induced by CCl4 treatment. Consistent with the serum AST and ALT activities, pre-treatment with Centaurea incana for 4 weeks to the rats resulted in a significant decrease in the concentration of hepatic MDA when compared with the CCl4 group. Conclusion: Our investigation provided convincing data that Centaurea incana decreases the lipid per-oxidation and liver enzymes, and increases the anti-oxidant defense system activity in the CCl4-treated rats. The mechanisms underlying hepatoprotection of the methanolic extract of Centaurea incana may be related to both its radical scavenging properties and indicate effects as a regulator of antioxidative systems.

Biography :

Email: khelifi_t_fatima@yahoo.fr