Angiotensin converting enzyme inhibitory effect of Ficus deltoidea jack standardised leaf extracts

Medicinal Chemistry

ISSN: 2161-0444

Open Access

Angiotensin converting enzyme inhibitory effect of Ficus deltoidea jack standardised leaf extracts

2nd International Conference on Medicinal Chemistry & Computer Aided Drug Designing

October 15-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA

Armaghan Shafaei, Abdalrahim F.A. Aisha, Nahdzatul Syima Muslim, Zeyad D. Nassar, ZhariIsmail1 and Amin Malik Shah Abdul Majid

Accepted Abstracts: Med chem

Abstract :

Ficus deltoidea jack (Moraceae) is a traditional medicinal plant which has been used for centuries for its health benefits such as antioxidant activity, improving the blood circulation and anti- hypertension effect. Angiotensin converting enzyme (ACE) inhibitors are considered as safe and effective antihypertension agents. Thus, research on finding new ACE inhibitors can provide safe and powerful anti-hypertension drugs. Methanolic (FD-M) and water (FD-W) extracts of F. deltoidea were screened for in vitro antihypertensive effect using an enzymatic-based ACE assay and by quantifying ACE concentration in umbilical vascular endothelial cells (HUVEC) lysates. The extracts were standardized by developing a reverse phase high performance liquid chromatography (RP-HPLC) for determination of the L-citrulline and L-arginine content. The extracts were also standardized for their primary and secondary metabolites including the total flavonoids, polyphenols, tannins, polysaccharides and glycosaponins. The effective RP-HPLC method with UV detection that involved precolumn derivatisation with O-phthaladehyde has been successfully developed and validated. Both extracts showed significant ACE inhibitory effect (P<0.05). In the enzymatic assay, FD-M showed the highest inhibition with IC 50 value 19.15 μg/mL. Likewise, FD-M and FD-W extracts of plant inhibited ACE expression in HUVECs by >60%.FD-Mextractwas found to contain relatively high L-citrulline, polyphenols, flavonoids, tannins and saponins content, while FD-W extract showedrelatively high L-arginine and polysaccharides content. The ACE inhibitory activity observed in F. deltoidea extracts support their traditional use asantihypertension treatment. This activity may be attributed to their high content of primary and secondary metabolites.

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