An unbiased, weighted network intersects with experimental approach to define the functions of PSMD10, an assembly chaperone of the proteasome

6^th International Conference on Bioinformatics & Systems Biology

August 22-23, 2016 Philadelphia, USA

Prasanna Venkatraman, Indrajit Sahu, Padma Nanaware, Meenal Mane and Soumen Roy

Tata Memorial Centre, India
Bose Institute, India

Posters & Accepted Abstracts: J Comput Sci Syst Biol

Abstract :

PSMD10, a proteasomal assembly chaperone, when overexpressed induces gene expression changes in HEK293 cells. A weighted network approach was employed to gain biological insights from these differentially expressed genes. Key network properties such as degree centrality and edge betweenness were identified. The top 30 edges (interactions) indicated that PSMD10 might be involved in cell fate decisions that commits stem cells to differente into neurons and/or astrocytes. Pathway analysis of the upregulated genes indicated a strong signature for the activation of neuroactive ligand receptor interaction pathway and Wnt signaling. Two hypothesis emerged from these comprehensive analysis: The presence of a neuronal differentiation program that includes genesis of astrocyte and neurons regulated by the source node PSMD10 and; the probability that PSMD10 may skew cell fate decision towards the formation of neurons. We tested these possibilities and provide preliminary and yet a compelling experimental evidence for the involvement of PSMD10 in the preferential differentiation of a neural progenitor cell line into neurons.

Biography :

Email: vprasanna@actrec.gov.in