A study on the effect of cimetidine and L-carnitine on myoglobinuric acute kidney injury in male rats

4^th International Conference on Nephrology & Therapeutics

September 14-16, 2015 Baltimore, USA

Suzanne Albert Naguib Estaphan

Cairo University, Egypt

Posters-Accepted Abstracts: J Nephrol Ther

Abstract :

Rhabdomyolysis-induced myoglobinuric acute renal failure accounts for about 10-40% of all cases of acute renal failure (ARF). Iron, free radicals, nitric oxide and Cytochrome P 450 are involved in the pathogenesis of myoglobinuric ARF. The aim of this study was comparing the effect of cimetidine, L-carnitine and both agents on myoglobinuric ARF in rats. Forty rats were divided into 5 groups; group 1: Control rats. The remaining rats were injected with 50% glycerol (10 ml/kg, i.m.) and were divided into: Group 2: Myoglobinuric ARF, group 3: Received L-carnitine (200 mg/kg, i.p.), group 4: received cimetidine (150 mg/kg i.p) and group 5: received both agents together. 48 hours later, blood pressure was measured. 24 hours urine collection and blood samples were collected to evaluate GFR, BUN, creatinine, K, sodium, serum creatine kinase, plasma NO and glutathione levels. Kidney specimens were taken to investigate renal cytochrome P450 and for histopathological examination. Cimetidine treatment significantly decreased creatinine, BUN, K, Na, SBP and creatine kinase and increased GFR compared to group 2. L-carnitine exerted similar changes except the effect on K and GFR. NO was significantly decreased while renal glutathione and cytochrome P450 were significantly increased in groups treated with L-carnitine or cimetidine as compared to group 2. Combined treatment further improved renal functions, creatine kinase, oxidative stress parameters and SBP as compared to each therapy alone. The histological changes confirmed biochemical findings. Cimetidine and L-carnitine have protective effect - almost equally- against myoglobinuric renal failure. Using both agents together made the renal injury minimal.

Biography :

Email: sestaphan@kasralainy.edu.eg