Dye Agar | Open Access Journals

Journal of Textile Science & Engineering

ISSN: 2165-8064

Open Access

Dye Agar

Dye Agar plates were prepared using PDA containing 60 mg L-1 of each individual dye. The plates were inoculated with a 1 cm 2 piece of the fungus isolated from a six-day PDA plate growth, cut from the actively growing fungal culture. Color agar plates which were not inoculated served as controls. All plates were incubated at 28 ° C. After six days, the size of the discoloration halo was measured. An experimental setup and a measurement technique have been developed so that the diffusion in model foods (agar gels) can be visualized and quantified. The diffusion of aqueous solutions of varying concentrations of two dyes (rhodamine 6G and methylene blue) in agar gels was followed in situ at three temperatures (30, 50, 70 ° C) until equilibrium be reached. The nature of the diffusion process (in terms of the amount of dye diffused in the gel) was studied using image analysis techniques. The diffusion coefficient, D, was estimated using Fick's second diffusion law and was found to be ∼10−10 m2 s - 1. The effect of the size of the diffusing molecule, as well as that of the temperature Diffusion treatment has been studied and has been shown to be significant.


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