There is a growing interest in cell therapies using mesenchymal stromal cells (MSCs) to repair bone defects. MSCs have the ability to differentiate into osteoprogenitors and osteoblasts as well as to form a calcified bone matrix. However, the molecular mechanisms governing mineralization during osteogenic differentiation remain unclear. The non-collagen proteins in the extracellular matrix are thought to control different aspects of mineralization. Since osteocalcin is the most abundant non-collagenous bone matrix protein, the purpose of this study is to investigate the roles of osteocalcin in the production of mineral species during MSC osteogenesis. Using Raman spectroscopy, we found that the maturation of mineral species was affected by the level of expression of osteocalcin. After osteocalcin was eliminated, the maturation of the mineral species was delayed and the total hydroxyapatite was less than the control group. In addition, the expression of osteogenic marker genes, including RUNX2, alkaline phosphatase, collagen type I and osteonectin, was downregulated during osteogenic differentiation from the control group; while the gene expression of osterix was upregulated after the knockdown. Together, osteocalcin plays an essential role in the maturation of mineral species and modulates the osteogenic differentiation of MSCs. The results offer new perspectives on improving the formation of new bones, such as for the treatment of osteoporosis and healing of fractures.
Research Article: Journal of Bioengineering & Biomedical Science
Research Article: Journal of Bioengineering & Biomedical Science
Research Article: Journal of Bioengineering & Biomedical Science
Research Article: Journal of Bioengineering & Biomedical Science
Research Article: Journal of Bioengineering & Biomedical Science
Research Article: Journal of Bioengineering & Biomedical Science
Research Article: Journal of Bioengineering & Biomedical Science
Research Article: Journal of Bioengineering & Biomedical Science
Scientific Tracks Abstracts: Cardiovascular Diseases & Diagnosis
Scientific Tracks Abstracts: Cardiovascular Diseases & Diagnosis
Posters & Accepted Abstracts: Cancer Science & Therapy
Posters & Accepted Abstracts: Cancer Science & Therapy
Posters & Accepted Abstracts: Journal of Physiotherapy & Physical Rehabilitation
Posters & Accepted Abstracts: Journal of Physiotherapy & Physical Rehabilitation
Posters & Accepted Abstracts: Journal of Bioengineering & Biomedical Science
Posters & Accepted Abstracts: Journal of Bioengineering & Biomedical Science
Journal of Bioengineering & Biomedical Science received 307 citations as per Google Scholar report
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