Journal of Bioengineering & Biomedical Science

Mitsuo Umezu

Publications

• Research Article
  Tissue Engineered Trachea Using Decellularized Aorta
  Author(s): Ana C. Paz, Koji Kojima, Kiyotaka Iwasaki, Jason D. Ross, Jose A. Canseco, Mitsuo Umezu and Charles A VacantiAna C. Paz, Koji Kojima, Kiyotaka Iwasaki, Jason D. Ross, Jose A. Canseco, Mitsuo Umezu and Charles A Vacanti
  
  Background: Several approaches for the development of tracheal substitutes for the treatment of extensive tissue defects have been explored over the years. However, a completely satisfactory approach has not been achieved. Previously, we described a composite tissue engineered trachea (TET) using chondrocytes seeded onto a polyglycolic acid (PGA) fiber-mesh. This study was considered to improve the design and functionality of the TET by using a porcine decellularized aorta as the scaffold. Methods: Chondrocytes were harvested from sheep tracheal cartilage and were suspended in medium. The chondrocytes were then seeded onto PGA and incubated in vitro for 1week. A 3x4 cm piece was cut from a decellularized aorta and four 0.5x3 cm pieces were excised from one side in a comb like fashion. A silicon stent was ins.. Read More»
  DOI: 10.4172/2155-9538.S2-001

  Abstract PDF