I am pleased to mention that during the year 2019, all issues of Volume 5 were published online well within the time and the print issues were also brought out and dispatched within 30 days of publishing the issue online.
Raghad Khalid Mwafaq*, Alsafa Nassr Khalaf Harbi, Shaimaa Khalid Moufak, Abeer Sajid Abd Ali, Alyaa Kareem Abood and Eman Ghazi Metaab
Background: Lysosomal Membrane-Associated Glycoprotein 3 (LAMP3) also known as cluster of differentiation CD63 is a member of tetraspanin, and a kind of membrane protein (III) is modified by N-glycosylation. It is found in lysosomes and is preferred in different types of cancer. Tumor stromal cells in the primary stage can induce tumorigenesis in numerous types of cancers. Also, stimulates metastatic growth by supplying the pre-metastatic niche. However, it can stimulate invasion and metastasis of tumor cells to other organs while maintaining survival and proliferation, therefore being effective in colonizing in metastatic sites. Objective of this study is to appreciate the expression of – LAMP3 in stromal cancer cells in patients with stomach cancer and detect the linkage between the expression of this protein and clinicopathological parameters, it is the first study in Iraq.
Methods: The total formalin-fixed paraffin-embedded blocks number of stomach tissue samples obtained was sixty. These blocks were divided into forty-five blocks as the patients’ group and fifteen blocks as the control group. The blocks were cut into histological sections and then discordant into two parts to stain by common histological hematoxylin and eosin and immunohistochemistry technique by using the LAMP3 marker kit.
Results: High score intensity of this marker was recorded in advanced stages (III and IV) in about 29 (64.44%) at score +4 in about 21 (46.6%) respectively. Also, concerning the age it was positively expressed in 11 (24.44%) of patients less or equal to 55 years. Conversely, to patients more than 55 years in about 30 (66.66%) as positive. The diffuse subtype was the highest positive to the marker in about 20 (44.44%). A significant association was seen in the grade of tumor as a positive expression to marker. Invasion depth was highly positively expressed in pT3 and pT4 about 75.55%, and 8.88%. Involving lymph nodes was a low expression of this marker at N0 which was recorded at 8.88%, whereas N1,2 and 3 positively expressed at about 91.11%. The advanced stage (TNM) high was recorded in about 64.44% at III and IV.
Conclusion: This marker might be a good marker for early diagnosis of stomach cancer patients in Iraq.
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi 