Journal of Microbiology and Pathology

Open Access

Current Issue

Volume 5, Issue 2 (2021)

    Research Article Pages: 1 - 4

    Analysis of the Dengue Infection, Occurrence and Hematological Profile of Dengue Patients in Dhaka City.

    Ekhlas Uddin

    In the tropical and subtropical area, dengue virus is mostly found. To the determination of the occurrence of dengue viral infection and hematological profile of dengue infected peoples in the Dhaka city. A sum of 255 samples of blood from both sex and different age groups were collected in this research. Dengue nonstructural protein 1 (NS1) and complete blood count (CBC) test were performed for the study. During this study period, total 255 samples were tested. Among these, 67samples were diagnosed as positive. Seroprevalence of dengue was 26.30%. Out of 67 positive dengue patients, 41 (61.2 %) were male and 26 (38.8 %) were female. Dengue infection was observed more in 21 to 30years age group followed by above 40years and 16 to 20years but observed less in of 1st day to 5years followed by 11 -15years, then 6 -10years and 31 - 40years. It was seen that the >40years of age group had the  maximum unit of cases having low platelet count followed by 21-30years and  it was also seen that 21-30years of age group had the maximum range of cases having low WBC count followed by above 40years of age group. In our research, we showed thrombocytopenia (100000 - 150,000/μl) with leucopenia (White Blood Cells, WBC <5000/μl) in 8(26.6%) cases and thrombocytopenia (100000-150,000/μl) without leucopenia in 9 (24.3%) cases but thrombocytopenia (<100000/μl) with leucopenia (White Blood Cells (WBC) < 5000/μl) in 5(16.7%) cases and thrombocytopenia (<100000/μl) without leucopenia in 6 (16.2%) cases. Among 67 positive cases, we found that (26.6%) had DF and (16.7%) had DHF according to WHO classification. For validating more reliability, this research needs further work.

    Research Article Pages: 1 - 4

    Evaluation of some Nodulation Genes found in Bambara Symbiotic Rhizobia Strains.

    Olaoluwa AJAYI

    Rhizobia are known to have specificity for their leguminous host . bambara groundnut although a legume has shown promiscuous ability nodulating with many Rhizobia spp. Bradhyrhizobia spp were recovered from selected Nigerian soils by trapping them in the nodules of Bambara groundnut(BG) using pot hose experiments. They were isolated from the nodules and authenticated in the screen house and on the field and six strains along-side USDA110 (control) were selected after they were found to be highly effective and efficient. The DNA was exyracted and Pcr was carried out to check for the presence of Nod A, NodB, NodzA and  NodzB genes. Nod A and Nod B which are conserved in all Rhizobia spp were found to be present. NodzA was found to be present in five of them while Nodz B was found to be present in all six of them. NodzA and Nodz B which are functional genes in soybean symbiointic Rhizobia strains this therefore suggests that BG symbiotic rhizobia strains may be able to nodulate soybean legumes and that NodzA and  Nodz B may possibly play a major role in the nodulation process of BG.

    Research Article Pages: 1 - 3

    Quantification of Total Protein and Related Enzymes in Fusarium wilt infected Lens culinaris medic.

    Anita Singh

    Quantification of protein and their related enzymes was studied in Lentil (lens culinaris medic) infected with Fusarium oxysporum F. Sp. lentis causing wilt disease. The contents of total protein and protease activities in healthy and diseased counter parts of lentils were measured. Different plant parts showed variation in their protein contents were recorded higher in wilt infected plant then healthy leaf, stem, fruit and seeds while protiase activities were recorded higher in normal leaf, stem, fruit and seeds as compared to infected plant. 

    Editorial Pages: 1 - 2

    Podocyte pathology and their techniques.

    Qiang Chen*

    Podocytes keep up the glomerular filtration obstruction, and the solidness of this boundary relies upon their exceptionally separated postmitotic aggregate, which likewise characterizes the specific weakness of the glomerulus. Ongoing podocyte science and quality disturbance concentrates in vivo show a causal connection between anomalies of single podocyte particles and proteinuria and glomerulosclerosis. Podocytes live under different burdens and neurotic upgrades. They adjust to look after homeostasis, however extreme pressure prompts maladaptation with complex natural changes including loss of uprightness and dysregulation of cell digestion. Podocyte injury causes proteinuria and separation from the glomerular storm cellar layer. Notwithstanding "debilitated" podocytes and their separation, our comprehension of glomerular reactions following podocyte misfortune needs to address the pathways from podocyte injury to sclerosis. Studies have discovered an assortment of glomerular reactions to podocyte brokenness in vivo, for example, disturbance of podocyte-endothelial cross talk and initiation of podocyte-parietal cell associations, all of which assist us with understanding the mind boggling situation of podocyte injury and its outcomes. This audit centers around the cell parts of podocyte brokenness and the versatile or maladaptive glomerular reactions to podocyte injury that lead to its significant outcome, glomerulosclerosis.

    Editorial Pages: 1 - 1

    Antimicrobial resistance in clinical studies

    Yongho Bae

    The rise and spread of medication safe microorganisms that have procured new opposition components, prompting antimicrobial obstruction, keeps on compromising our capacity to treat basic contaminations. Particularly disturbing is the quick worldwide spread of multi-and container safe microscopic organisms (otherwise called "superbugs") that cause diseases that are not treatable with existing antimicrobial prescriptions like anti-microbials.

    Volume 5, Issue 5 (2021)

      Research Article Pages: 1 - 11

      Antimicrobial Susceptibility and Minimum Inhibitory Concentration of Salmonella enterica Isolates from Chickens in Some Selected Local Governments of Yobe State

      Ahmed, Idriss Jajere*, Abubakar, Bukar Mustapha, Mailafia Samuel, Raji, Abiola Mashood and Ahmed, Ibrahim Jajere

      Salmonella of poultry are zoonotic microorganisms transmitted to humans and other animals via contact with infected poultry feces, meat, eggs and formites. This study was conducted to phenotypically characterize Salmonella enterica from samples collected from chickens presented for slaughter in some selected Local Government Areas of Yobe State, Nigeria, as well as carry out antimicrobial susceptibility and minimum inhibition concentration on the isolates. A cloacal swab and blood samples were collected and transported on ice pack to Veterinary Microbiology Laboratory, University of Maiduguri and analyzed for the presence of Salmonella enterica. Samples were then inoculated onto Xylose Lysine Deoxycholate agar for morphological identification of Salmonella blackish colonies. A total of 600 (300 cloacal swab and 300 blood), consisting (202 males, 98 female chickens, 150 local and exotic each) were randomly sampled in 16 weeks for the isolation of Salmonella enterica. The presumptive Salmonella isolates were further characterized using the MicrobactTM GNB 24E System kit, with 40 randomly selected presumptive isolates (8 from blood and 32 from cloacal swab) tested using Microbact 24E GNB Computerize system, with 10 samples found to be positive for Salmonella organisms out of which 9 (22.5%) were from cloacal swab and 1 (2.5%) from blood. All the blood samples were tested for haemagglutination using slide method, 255 were found to be positive, where agglutination was observed. Where as only 8 (2.7%) were positives after blood culture 8 (2.67%). Exotic chickens showed the highest resistance level of (35%) to commonly used antibiotics (Amoxixillin and Ampicillin). The isolates from exotic chickens are susceptible to Ciprofloxacin 11 (68.8%), Ofloxacin 10 (62.5%), Gentamicin 2 (12.5%), Levofloxacin and Erythromycin 6 (37.5%), while intermediate to Norfloxacin 5 (31.3%) and Amoxicillin 7 (43.8%) but were resistant to Ampicillin 6 (37.5%%), Cefuroxime 10 (62.5%) and Amoxicillin 4 (25.0%). The MIC was carried out on all the 10 Salmonella isolated that showed positive on microbact 24E computerized system. All the 10 isolates from microbact 24E computerized system showed susceptibility to amoxicillin, ofloxacin, and ciprofloxacin on MIC. The MIC of ofloxacin and ciprofloxacin was distributed within 0.00175–2 μg/ml each, and for amoxilin, the MIC ranged between 0.00175-3.00 μg/ml. It is therefore, concluded that Salmonella organisms phenotypically characterized in the study area had antimicrobial susceptibility to routinely used antimicrobial drugs. As a result, it is suggested that the medications with high susceptibility be used to treat poultry salmonellosis in the study area.

      Editorial Pages: 1 - 1

      Molecular Docking: Bioinformatics tool

      Sandeep Kaur Ravala*

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      Commentary Pages: 1 - 1

      Industrial fermentation Procedures

      Qiang Chen*

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      Volume 5, Issue 3 (2021)

        Editorial Pages: 1 - 1

        Microbial Applications in Human Welfare

        H.C.Yashavanth Roy*

        The branch of biology that deals with the study of organism square measure known as “Microbiology”. The region i.e. the organic phenomenon and abiotic part contains a kind of microorganisms that exhibit helpful activities. They embrace tiny protoctist, fungi, bacteria, protozoans, mycoplasmas and connected organisms. An outsized range of microbes facilitate human civilization through their helpful activities. These activities square measure either of domestic, industrial or business importance. Its many applied branches like medical biology, food biology, industrial biology, etc. Fungi are often single celled or terribly advanced cellular organisms. They’re found in precisely concerning any surroundings however most live to tell the tale the land, principally in soil or on stuff instead of in ocean or H2O.

        Editorial Pages: 1 - 1

        Cell pathology techniques concerned in tissue cultures

        Sheila Jain*

        Cell pathology is that the study of sickness exploitation the techniques of recent cell biology. The aim is to grasp however cellular and molecular mechanisms act throughout sickness processes. Cellular pathology is that the study of sickness in organs, tissues and cells. Histopathology and cytopathology are key diagnostic tests within the initial detection and identification of cancer and different diseases supported by fashionable molecular techniques. Adviser cellular pathologists are ready to offer data on prognosis and facilitate to fitly direct therapies in post diagnostic treatment. Speedy advances in vegetative cell biology and regenerative medication have opened new opportunities for higher understanding sickness pathological process and development of recent diagnostic, prognostic and treatment approaches.

        Editorial Pages: 1 - 1

        Approaches of Clinical veterinary Pathology

        [email protected]*

        Clinical pathology worries with the identification of illness supported the laboratory analysis of bodily fluids like blood, waste or cavitary effusions, or tissue aspirates victimization the tools of chemistry, life science, medical science and molecular pathology. Clinical pathologists are utilized in diagnostic pathology, veterinary and medical teaching, research, and conjointly the pharmaceutical business. Bridging the divide between natural and experimental diseases, the journal details the diagnostic investigations of natural and rising diseases of animals; reports experimental studies enhancing understanding of the mechanisms of specific processes along with cancer, infection, medical science, metabolic and genetically mediate diseases.

        Editorial Pages: 1 - 1

        Plant Microbes associated in soil microorganisms

        Qin Fu*

        Plant growth and development involves a good coordination of the spacial and temporal organization of organic process, cell enlargement and cell differentiation. Orchestration of those events needs the exchange of signal molecules between the foundation and shoot, which might be laid low with each organic phenomenon and abiotic factors. Plants turn out a large vary of organic compounds as well as sugars, organic acids and vitamins, which might be used as nutrients or signals by microorganism populations. The final biological process pattern in plants depends on indeterminate growth and unvaried organogenesis, characterised by continued organic process within the meristematic regions.

        Editorial Pages: 1 - 1

        Molecular-biology applications in rhetorical

        Kin Hu*

        One of the foremost basic techniques of biological science to check macromolecule operate is molecular biological research. During this technique, deoxyribonucleic acid committal to writing for a macromolecule of interest is cloned exploitation enzyme chain reaction (PCR), and/or restriction enzymes into an inclusion (expression vector). A vector has three distinctive features: AN origin of replication, a multiple biological research web site (MCS), and a selective marker typically antibiotic resistance. Situated upstream of the multiple biological research area unit the promoter regions and therefore the transcription begin site that regulate the expression of cloned factor. This inclusion is inserted into either microorganism or animal cells. Introducing deoxyribonucleic acid into microorganism cells is done by transformation via uptake of naked deoxyribonucleic acid, conjugation via cell-cell contact or by transduction via microorganism vector.

        Volume 5, Issue 1 (2021)

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