Postdoctoral Research Fellow
Columbia University Medical Center, New York, USA
Riddhi Shah is currently Postdoctoral Research Fellow of the American Heart Association and the research is conducted in Columbia University Medical Center,USA. She has a biological research experience of Columbia University Medical Center, Department of Medicine, New York 09/12/2016 -06/30/2017 Postdoctoral Research: The effects of Sleep Restriction on Endothelial Activation in Pre- and Post-Menopausal Women • Assessing in endothelial cell harvesting biopsy and blood sample collection. • Isolation of circulating endothelial progenitor cells (EPC) and endothelial apoptotic microparticles (EMP). • Assessing EPC and EMP levels by flow cytometry • Isolating platelets and investigating for platelet activation and aggregation by flow cytometry. • White blood cell (WBC) isolation • HUVEC cell culturing and passaging. • Conducting hypoxia experiments on HUVEC cells
• Conducted PCR mutagenesis (Site-specific and random) on iCre recombinase gene cassette followed by cloning into the expression vector. • Tested the expression vector activity with reporter plasmids by Bacterial Solid and Liquid Culture Transformation. • Analyzed variants with routine and gradient PCR reactions (Taq, Q5, KOD, Pfu). • Constructed the TALE – DNA Binding Domain. • Modified the recombinase variants by binding them to TALE to increase their efficiency. • Constructed reporter CHO model cell line to test recombinase expression vectors. • Optimized transfection protocols in CHO and HEK 293 mammalian cells. • Confirmed the activity of the variants in CHO and 293HEK cell lines with transfection reagents. ((Fugene, DNA-In, Lipofectamine, Polyfect, K2-Reagent) • Optimized reportor and expression plasmid concentrations for efficient recombination and integration activities in CHO and 293HEK cells. • Performed Genomic and Total DNA isolation to test the integration and replacement of genes in the genome. Further confirmed using Southern Blotting. • Tested the CRISPR/gRNA setup for Flp variants in 293HEK cells to observe integration activity.