Journal of Infectious Diseases and Medicine

Department of Human Mucosal Vaccinology, Chiba University Hospital, Chiba 260-8670, Japan

Publications

• Mini Review   
  Probiotics can help Deliver Nanotechnology that Fights Against Norovirus Infection
  Author(s): Lennart Marcotte*
  
  Passive administration of negativing antibodies (Abs) is an seductive strategy for the control of gastrointestinal infections. still, an unanswered practical concern is the need to assure the stability of sufficient quantities of orally administered negativing Abs against intestinal pathogens (e.g., norovirus) in the harsh terrain of the gastrointestinal tract. To this end, we expressed a single- sphere Ab (VHH, nanobody) against norovirus on the cell face of Lactobacillus, a natural and salutary commensal element of the gut microbiome. First, we used intestinal epithelial cells generated from mortal convinced pluripotent stem cells to confirm that VHH 1E4 showed negativing exertion againstGII.17 norovirus. We also expressed VHH 1E4 as a cell- wall–anchored form in Lactobacillus paracasei BL23. Flow cytometry verified the expression of VHH 1E4 on the face of .. Read More»
  DOI: 10.37421/2576-1420.2022.7.271

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