Department of Chemistry, College of Computational and Natural Science, Kabridaher University, Kabri Daher, Ethiopia
Research Article
Determination of Levels of Aflatoxin in Maize Produced from Shebelle Zone, Somali Regional State, Eastern Ethiopia Using UHPLC
Author(s): Kebede Mamo*, Yared Shewarega and Aklilu Melese
Aflatoxin producing substances are extremely dangerous to humans and can result in cancer when taken in foods contaminated with
aflatoxin. In this study, the levels of aflatoxins (B1, B2, G1, and G2) in samples of maize collected from several Shebelle Zone districts
were quantified. It was examined using a fluorescence detector and UHPLC. The extraction solvents (80:20 v/v percent) were methanol
and water. Standard solutions of aflatoxins between 0.5 and 7 g/kg (ppb) demonstrated strong linearity from the calibration curve with
regression coefficient (R2) values of >0.9989. The spiked sample average percentage recoveries ranged from 89.78 to 97.87 percent. The
average total aflatoxin values in the maize samples from K1, K2, and K3 were 0.14, 356.19, and 174.05 g/kg, respectively. Except for K1,
all of the investigated maize samples had aflatoxin levels .. Read More»
DOI:
10.37421/2150-3494.2023.14.330
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