Journal of AIDS & Clinical Research

Yenisey Alfonso

Publications

• Research Article
  Multiplex PCR to Detect T. gondii Infection based on B1 Gene and 529 bp Repetitive Element
  Author(s): Yenisey Alfonso, Jorge Fraga, Zhaily Gonzalez, Narciso Jimenez, Yainais Borrero, Raymundo Cox, Carlos Fonseca, Francisco Bandera, Virginia Capo and Dora GinorioYenisey Alfonso, Jorge Fraga, Zhaily Gonzalez, Narciso Jimenez, Yainais Borrero, Raymundo Cox, Carlos Fonseca, Francisco Bandera, Virginia Capo and Dora Ginorio
  
  Polymerase Chain Reaction (PCR) has made a significant improvement in the diagnosis of toxoplasmosis. Nevertheless, a wide variety of targets and primers has been evaluated in different independent assays, and only few comparative studies have been performed. Even when the B1 gene and the 529 repetitive element have been the most studied markers, there no concluding remarks respect the best. Objetive: The aim of the present study was to design a multiplex PCR assay to simultaneously detect these two markers in a single multiplex PCR to diagnose T. gondii infection. Methods: Specific PCR primers targeting the B1 gene and 529 repetitive element were evaluated. After a careful optimization of multiplex PCR process, analytical sensitivity and specificity were evaluated. The usefulness of multiplex assay was evaluated using DNA from 8 T. gondii referenc.. Read More»
  DOI: 10.4172/2155-6113.1000435

  Abstract PDF