Metabolomics:Open Access

Wang H

Publications

• Research Article
  Consistency of Metabolite Determination from NMR Spectra over Time and Between Operators
  Author(s): Tso V, Eisner R, Macleod S, Ismond KP, Foshaug RR, Wang H, Joseph R, Chang D, Taylor N and Fedorak RNTso V, Eisner R, Macleod S, Ismond KP, Foshaug RR, Wang H, Joseph R, Chang D, Taylor N and Fedorak RN
  
  With the recent increase in publications of metabolomic-based studies, it is apparent that there are considerable differences in findings between laboratories. Even within a single centre, variability introduced by different highly skilled operators contributing to the same experiment can alter the results. These inconsistencies may contribute to unforeseen experimental confounders and, as such, represent a critical barrier to metabolomics based studies. This issue can impact studies throughout the research spectrum, from basic science to clinical research. One of the potential sources of unforeseen variance in many metabolomic-based studies occurs during the identification and quantification of metabolites. In our study, we examined the profiling consistency of NMR spectra as essential to the interpretation and analysis of metabolite concentrations. This .. Read More»
  DOI: 10.4172/2153-0769.1000151

  Abstract PDF