Journal of Bioanalysis & Biomedicine

Sameen Laeeq

Publications

• Research Article
  Sodium Deoxycholate Micelles Activated Separation of Coexisting Fivenucleobases by High-performance Thin-layer Chromatography
  Author(s): Ali Mohammad, Sameen Laeeq and Abdul MohemanAli Mohammad, Sameen Laeeq and Abdul Moheman
  
  A novel high-performance thin-layer chromatographic method has been developed for the resolution of fi ve-coexisting nucleobases (adenine, guanine, cytosine, thymine, and uracil). The nucleobases were separated on aluminum-backed cellulose 60 F 254 plates with the aid of 5.0% aqueous sodium deoxycholate (NaDC)-acetonitrile (AcN), 1:3 (v/v) as mobile phase. All the nucleobases were viewed on HPTLC plates under 254nm UV light. The order of R F value given in parentheses was guanine (0.12) < adenine (0.44) < cytosine (0.50) < uracil (0.72) < thymine (0.84). The effect of pH (acidity or basicity) of the mobile phase on the retention of individual nucleobases was examined. Furthermore, the effect of interference of mono- (Li + , Na + ), and bivalent (Mg 2+ , Ba 2+ , Co 2+ , Ni 2+ , Cu 2+ , Zn 2+ , Cd 2+ , Pb 2+ ) cations; mono- .. Read More»
  DOI: 10.4172/1948-593X.1000022

  Abstract PDF