Journal of Bioanalysis & Biomedicine

Robert Bases

Publications

• Research Article
  Enhanced Content of IgG in Burkitt’s Lymphoma Cells after Treatment with the Topoisomerase II Inhibitor, Lucanthone
  Author(s): Robert Bases, Rukmani Lekhraj, Xudong Tang, Jinghang Zhang, Zhi Duan, Jennifer T Aguilan and Edward NievesRobert Bases, Rukmani Lekhraj, Xudong Tang, Jinghang Zhang, Zhi Duan, Jennifer T Aguilan and Edward Nieves
  
  Burkitt’s lymphoma cells (CRL-1647) which had survived treatment with lucanthone contained 3.6 fold more IgG than untreated cells, although most of the cellular immunoglobulins were still IgM. DNA activation induced cytidine deaminase (AID) was increased 5 fold in these surviving cells, consistent with active Class Switch Recombination (CSR). Progeny of the small fraction of cells which had survived 20 h exposure to 8 μM lucanthone before rescue were cloned. 1.5 × 108 cloned cells contained ~1 μg of cytidine DNA deaminase, as determined from affinity column isolation of the enzyme, assayed by digestion of a 30 nt 32P labeled specific DNA substrate. Before lucanthone treatment, little AID could be detected. After the second treatment, a six fold increase in AID was found. In confirmation, Western blot determinations of AID from lysates of lucanthone treated .. Read More»
  DOI: 10.4172/1948-593X.1000176

  Abstract PDF