Cardiovascular Diseases & Diagnosis

Raman Purushothaman

Publications

• Research Article
  Increased Myeloperoxidase Expression is Associated with Increase in Intra-Plaque Hemorrhage, Iron Content, Inflammation and Neovascularization in Diabetic Atherosclerosis: Implications for Plaque Progression
  Author(s): Raman Purushothaman, Carlos L. Alviar, Arthur Tarricone, Prakash Krishnan, Annapoorna Kini, Samin Sharma, Valentin Fuster and Pedro. R. MorenoRaman Purushothaman, Carlos L. Alviar, Arthur Tarricone, Prakash Krishnan, Annapoorna Kini, Samin Sharma, Valentin Fuster and Pedro. R. Moreno
  
  Background: Myeloperoxidase (MPO), a heme peroxidase enzyme produced by macrophages, is a marker of oxidative stress associated with increased cardiovascular events in Diabetes Mellitus (DM). In this study we hypothesize that MPO protein and gene expression is associated with increased Intra-Plaque Hemorrhage (IPH), iron deposition, plaque inflammation and neovascularization in DM atheroma. Methods: Twenty-six human aortas with advanced plaques (14 DM vs.12 No-DM) were studied. MPO protein and gene expression were quantified with immunohistochemistry and RT-PCR respectively. IPH was evaluated using H&E stain. Plaque iron was scored using Perl’s stain and graded Inflammatory cells and neovessels were manually counted in parallel sections using with (CD68 and CD3), and (CD34) using immunohistochemistry method. Resul.. Read More»
  DOI: 10.4172/2329-9517.1000118

  Abstract PDF