Journal of Bioanalysis & Biomedicine

Nayanabhirama Udupa

Publications

• Research Article
  High Performance Liquid Chromatographic Method for the Determination of Clobetasol in Rat Plasma and its Application to Skin Penetration
  Author(s): Prashant B. Musmade, Praful B. Deshpande, Swapnil J. Dengle, Kranti B. Talole, Shriram M. Pathak, Adiga MNS, Krishnamurthy Bhat, Nayanabhirama Udupa and K. Laxminarayana BairyPrashant B. Musmade, Praful B. Deshpande, Swapnil J. Dengle, Kranti B. Talole, Shriram M. Pathak, Adiga MNS, Krishnamurthy Bhat, Nayanabhirama Udupa and K. Laxminarayana Bairy
  
  A simple a nd sensitive high per for ma nce liquid chromatography (HPLC) method was developed for qua ntification of clobeta sol (CLB) in r a t pla sma . Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C 18 (250.0 × 4.6 mm, 5 μ m particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was va lida ted a s per the United States Food and Dr ug Administration (USFDA) guideline. This method was l.. Read More»
  DOI: 10.4172/1948-593X.1000013

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