Journal of Bioanalysis & Biomedicine

Moses Prince Francis

Publications

• Research Article
  Simple and Validated Method for Estimation of Amlodipine by LC-MS (ESI) Using Healthy Indian Human Volunteers: and Evaluation of Pharmacokinetic Parameters
  Author(s): Moses Prince Francis, Selvadurai Muralidharan, Sekar Rajan, Nagarajan and Bhojraj SureshMoses Prince Francis, Selvadurai Muralidharan, Sekar Rajan, Nagarajan and Bhojraj Suresh
  
  A simple and validated liquid chromatographic–mass spectrometric method (LC-MS) for amlodipine in human plasma was quanti fi ed using LC-MS (ESI). Chromatography was performed on a C 18 analytical column, the mobile phase used was Acetonitrile-10mM Ammonium acetate in the ratio of 90:10%v/v and the retention times were 0.829 and 1.281 min for azithromycin (Internal standard) and amlodipine respectively. The ionization was optimized using ESI (+) and enhanced selectivity was achieved. The method is validated as per FDA guidelines. The analyte was shown to be stable over the timescale of the whole procedure. The pharmacokinetic parameters such as peak plasma concentration (C max ), Time to peak Concentration (t max ), Area under the plasma concentration-time curve (AUC 0-t & AUC 0- ∞ ), elimination rate constant (K eli ), Elimin.. Read More»
  DOI: 10.4172/1948-593X.1000025

  Abstract PDF