Journal of Bioengineering & Biomedical Science

Manoj Kumar

Publications

• Research Article
  Ectopic Overexpression of Lectin in Transgenic Brassica juncea Plants Exhibit Resistance to Fungal Phytopathogen and Showed Alleviation to Salt and Drought Stress
  Author(s): Deepak Kumar, Shashi Shekha, Sandip Bisht, Vivek Kumar, Ajit Varma, Manoj Kumar and Mukesh SaxenaDeepak Kumar, Shashi Shekha, Sandip Bisht, Vivek Kumar, Ajit Varma, Manoj Kumar and Mukesh Saxena
  
  Lectins are ubiquitous in nature and one of the most important secondary metabolites in plants. They are heterogeneous class of proteins that bind specifically and reversibly to carbohydrates and are used as a defence tool against various abiotic and biotic stresses. In this study, we describe generation of transgenic Brassica juncea plants over-expressing chickpea lectin in conferring fungal resistance. In addition, the transgenic plants also showed resistance against salinity (NaCl) and mannitol induced drought stress. The stable integration and transcript expression of the transgene was confirmed by southern blot and RT-PCR analysis in the transgenic plants. The transgenic lines were evaluated for resistance against Alternaria brassicae as assessed by the number and size of lesions and time taken for leaf necrosis, the Alternaria infection was delayed and restricted in the transgen.. Read More»
  DOI: 10.4172/2155-9538.1000147

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