Journal of Bioengineering & Biomedical Science

Krishna Dasa SS

Publications

• Research Article
  The nature of floating cells in human embryonic stem cell culture
  Author(s): Chen L, Jin Q, Gong J and Krishna Dasa SSChen L, Jin Q, Gong J and Krishna Dasa SS
  
  A consistent presence of floating cells is a common phenomenon in cultures of human embryonic stem cells (hESCs). However, little attention has been paid to their existence. It is currently believed that unavoidable imperfections in culture conditions lead the cells to undergo senescence and apoptosis resulting in unattached cells floating in the culture medium. Inspired by recent studies on mitotic activities in human embryonic stem cell colonies, we believe the existence of floating cells is not simply the result of unfavorable growth conditions but an intrinsic phenomenon resulted from maintaining the pluripotency of hESCs under the culture conditions. We tested this hypothesis with a set of systematic experiments and discovered: 1) the ratio of floating cells to attached cells was significantly increased with culture time; 2) the number of floating cells could be manipulated. For .. Read More»
  DOI: 10.4172/2155-9538.1000129

  Abstract PDF