Journal of Nephrology & Therapeutics

Ken Sakai

Publications

• Special Issue Article
  ACE2- Ang 1-7-MAS Axis in Human Diabetic Nephropathy
  Author(s): Sonoo Mizuiri, Yoshiko Nishizawa, Makoto Hamanoue, Hiromichi Hemmi, Michitsune Arita, Kazutoshi Shibuya, Toshiyuki Aoki, Yasushi Ohashi, Ken Sakai and Atsushi AikawaSonoo Mizuiri, Yoshiko Nishizawa, Makoto Hamanoue, Hiromichi Hemmi, Michitsune Arita, Kazutoshi Shibuya, Toshiyuki Aoki, Yasushi Ohashi, Ken Sakai and Atsushi Aikawa
  
  The angiotensin-converting enzyme 2 (ACE2)- angiotensin-(1-7) [Ang (1-7)]-MAS receptor (MAS) axis might act as a counter-regulatory system against the angiotensin-converting enzyme (ACE)- angiotensin II (Ang II)-AT1 receptor (AT1) axis. We studied renal ACE/ACE2-Ang 1-7-MAS expression in human diabetic nephropathy. The subjects were 17 diabetic nephropathy (DN) patients, 17 healthy kidney donors, and 11 minimal change nephrotic syndrome (MCNS) patients as disease controls. Double immunofluorescent staining of kidney sections for ACE and ACE2, Ang 1-7 and ACE2, or MAS and ACE2 was performed, and the results were observed by confocal microscopy. For MAS, immunostaining, in situ hybridization, and RT-PCR were also performed. The percentage area that was positively immunostained for MAS and the intensity of the staining were evaluated by computerized im.. Read More»
  DOI: 10.4172/2161-0959.S2-005

  Abstract PDF