Journal of Computer Science & Systems Biology

Jiawen Zhu

Publications

• Research Article
  An Integrated Method for Detecting Micro RNA Target Proteins through Reverse-phase Protein Arrays
  Author(s): Jiawen Zhu, Song Wu and Jie YangJiawen Zhu, Song Wu and Jie Yang
  
  Objective: Understanding functions of microRNAs (or miRNAs), particularly their effects on protein degradation, is biologically important. Emerging technologies, including the reverse-phase protein array (RPPA) for quantifying protein concentration and RNA-seq for quantifying miRNA expression, provide a unique opportunity to study miRNA-protein regulatory mechanisms. One naïve way to analyze such data is to directly examine the correlation between the raw miRNA measurements and protein concentrations estimated from RPPA. However, the uncertainty associated with protein concentration estimates is ignored, which may lead to less accurate results and significant power loss. Methods: We propose an integrated nonlinear hierarchical model for detecting miRNA targets through original RPPA intensity data. This .. Read More»
  DOI: 10.4172/jcsb.1000166

  Abstract PDF