Human Genetics & Embryology

Hexin Chen

Publications

• Research Article
  Enrichment and Selective Targeting of Cancer Stem Cells in Colorectal Cancer Cell Lines
  Author(s): Shunqi Wang, Deepak Kanojia, Pang-kuo Lo, Varun Chandrashekaran, Xinrui Duan, Franklin G Berger, Qian Wang and Hexin ChenShunqi Wang, Deepak Kanojia, Pang-kuo Lo, Varun Chandrashekaran, Xinrui Duan, Franklin G Berger, Qian Wang and Hexin Chen
  
  Cancer stem cells (CSCs) are the subpopulation of cells within a tumor proposed to be responsible for tumor initiation, relapses, and resistance to chemotherapeutic drugs. Here we optimized sphere culture conditions to isolate and enrich CSCs from colorectal cancer cell line IMCE-Ras. Spheroid cells that developed in culture expressed high levels of putative stem cell markers, and showed stronger anchorage-independent growth abilities and resistance to conventional chemotherapeutic drugs compared with the initial monolayer adherent cells. Xenograft transplantation assays further demonstrated that IMCE-Ras spheroid cells are highly enriched in CSCs. To develop CSC-targeted therapy, we found that the relative percentage of CSC in IMCE-Ras cells was significantly decreased after a short duration exposure to DNA Methylation inhibitor 5-aza-2’-deoxycytidine (5-Aza-dC), indicating tha.. Read More»
  DOI: 10.4172/2161-0436.S2-006

  Abstract PDF