Journal of AIDS & Clinical Research

Hao Wu

Publications

• Research Article
  Application of Ambiguous Analysis for Determining Recent Infections of HIV-1 in a China Men Who Had Sex with Men Population
  Author(s): Xiaoyan Bai, Hongwei Zhang, Wei Shao, Lan Li, Zheng Zeng and Hao WuXiaoyan Bai, Hongwei Zhang, Wei Shao, Lan Li, Zheng Zeng and Hao Wu
  
  Objective: To distinguish HIV-1 recent infection (≤ 300 days) from long-term HIV-1 infection (>300 days) in China men who had sex with men (MSM) population. We analyzed the change over time in the proportion of ambiguous nucleotides in patient plasma sequences. We hypothesized that this method could be used to determine recent infections. Methods: HIV-1 sequences and clinical data of MSM were collected from June 2007 to September 2010. All the sequences were obtained by single genome amplification and sequencing. HIV-ambi-count was used to calculate the proportion of ambiguous nucleotides, and the optimal cut-off values to identify recent infection were established using receiver operator characteristic analysis (ROC). Results: A total of 188 sequences from 150 patients were collected (38 patients were sampled twice at different time points), consisting of 118 se.. Read More»
  DOI: 10.4172/2155-6113.1000279

  Abstract PDF