Journal of Genetics and Genomes

ISSN: 2684-4567

Open Access

Amit Kumar


  • Research Article
    Isothermal Nucleic Acid Amplification System: An Update on Methods and Applications
    Author(s): Sasi Kumar, Amit Kumar and Gnanavel VenkatesanSasi Kumar, Amit Kumar and Gnanavel Venkatesan

    Polymerase chain reaction is the most commonly used nucleic acid amplification for the diagnosis of several infectious and non-infectious diseases of animal and humans. But, it requires high expensive instrumentation for amplification and analysis. There are several isothermal amplification methods, such as Nucleic Acid Sequence Based Amplification (NASBA), Strand Displacement Amplification (SDA), Isothermal Multiple Displacement Amplification (IMDA), Rolling Circle Amplification (RCA), Helicase-Dependent Amplification (HDA), Single Primer Isothermal Amplification (SPIA), signal-mediated amplification of RNA technology, circular helicase dependent amplification, Recombinase Polymerase Amplification (RPA), Polymerase Spiral Reaction (PSR) and Loop-mediated Isothermal Amplification (LAMP). These methods can be amplified at a constant temperature by a simple water bath or heating block w.. Read More»

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