Journal of Computer Science & Systems Biology

Alessandro Ambrosi

Publications

• Research Article
  Vectors and Integration in Gene Therapy: Statistical Considerations
  Author(s): Alessandro Ambrosi and Clelia Di SerioAlessandro Ambrosi and Clelia Di Serio
  
  In gene therapy the integration process of the viral DNA genome into the host cell genome is a necessary step for virus integration. Just few years ago, retrovirus integration was believed to be random and the chance of accidentally activating a gene was considered remote. It has been seen that this process is not random and different viruses may show different preferences to integrate in some specific areas of the genome. Tumorigensis associated to some studies in gene therapy is suspected to be caused by insertion process. Depending on whether the provirus integrates into or in the vicinity of genes (Transcription Start Sites , TSS), normal trascription can be enhanced or disrupted thus inducing oncogenic mutations. This is called “insertional mutagenesis”. Investigating whether an area over the genome could be favoured by retrovirus integrat.. Read More»
  DOI: 10.4172/jcsb.1000023

  Abstract PDF