Journal of Pharmacognosy & Natural Products

Plants have historically been used for treatment of human and placental mammal ailments in Federal Democratic Republic of Ethiopia by totally different ethnic and social teams. However, this valuable supply of data isn't adequately documented, that impedes their widespread use, analysis and validation. Here, we have a tendency to recorded autochthonic information and commonplace practices for human and placental mammal unwellness management, of 3 ethnic teams (Aari, Maale and Bena-Tsemay) in South Omo Zone of Southern Nations, Nationalities and Peoples Regional State, Ethiopia. A cross-sectional study was meted out employing a semi-structured form to document information of fifty ancient healers (40 male and ten female) in medicative plant use for treatment of human and placental mammal ailments. Descriptive statistics were wont to analyze and summarize the ethno-botanical knowledge

Chromatographic separation of equal ratio of CH₂Cl₂-MeOH extract of the stem barks of Podocarpus falcatus led to the isolation of three compounds namely: 4β-carboxy-19-nor-totarol (1), β-sitosterol (2), 4-hydroxybenzoic acid (3) and (E)-methyl-3, 4, 5-trimethylhex-2-enoate (4). The structures of the compounds were established based on the analysis of 1D and 2D NMR spectroscopic data. These compounds were reported from this plant for the first time. The crude extract and isolated compounds were evaluated for their antibacterial activity using disk diffusion assay method. The crude extract showed a strong activity against S. aureus. Compounds (1) and (2) showed a relatively moderate activity against S. flexineri and S. typhimurium respectively, whereas, compound (3) and (4) demonstrated a strong activity against S. aureus. The crude extract and the isolated compounds showed antibacterial activity as compared to the reference gentamycin indicating that this plant has potentially antibacterial properties.