Journal of Surgery

Context and objective: The production of ceramide-1-phosphate, which is catalysed by ceramide kinase (CerK), may influence a number of cellular processes, including inflammation and cell division. Here, we looked at how the newly created CerK inhibitor NVP-231 affected the viability and proliferation of cancer cells, as well as the function of cell cycle regulators linked to these reactions.

Experimental methodology: Gradually increasing concentrations of NVP-231 were applied to the lung cancer cell line NCI-H358 and the breast cancer cell line MCF-7 in order to measure DNA synthesis, colony formation, and cell death. The cell cycle distribution of the cells was analyzed using flow cytometry, and alterations in the expression and activation of cell cycle regulators were found using Western blot analysis.

Important findings: NVP-231 decreased DNA synthesis, colony formation, and cell survival in both cell lines in a concentration-dependent manner. Additionally, it caused apoptosis, as seen by elevated caspase-3 and caspase-9 cleavage and DNA fragmentation. NVP-231 reduced the proportion of cells in the S phase and caused an M phase arrest with an elevated mitotic index, as shown by increased histone H3 phosphorylation, according to cell cycle analysis. When staurosporine was added to NVP-231 therapy, the impact on the cell cycle became even more noticeable. Lastly, siRNA-induced down-regulation of CerK sensitized cells to staurosporine-induced apoptosis, although overexpression of CerK protected against this process.

Conclusions and significance: our findings reveal for the first time the critical function that CerK plays in the regulation of the M phase in cancer cells. We propose that CerK be specifically inhibited, with the use of medications like NVP-231, in conjunction with standard pro-apoptotic treatment.

Surgery, often regarded as the pinnacle of medical intervention, embodies a delicate balance between meticulous science and intuitive artistry. In this article, we delve into the intricate relationship between the technical precision demanded by surgical procedures and the humanistic touch required for compassionate care. Through exploring the evolution of surgical practices, the integration of advanced technologies, and the essential role of empathy, we illuminate the multifaceted nature of surgery as both an art and a science.

Aesthetic reconstructive surgery represents a remarkable intersection of artistry and medical science, where innovative techniques are employed to restore not only physical appearance but also psychological well-being. In this article, we explore the evolution of aesthetic reconstructive surgery, highlighting advanced techniques used to address a wide range of conditions, from congenital anomalies to traumatic injuries and postsurgical deformities. Through a lens of compassion and precision, we delve into the transformative impact of these procedures on patients' lives, emphasizing the holistic approach that considers both the aesthetic and functional aspects of reconstruction. Keywords: aesthetic reconstructive surgery, plastic surgery, reconstructive techniques, cosmetic enhancement, patient satisfaction.

Reconstructive surgery has witnessed remarkable advancements in recent years, revolutionizing the field and offering new hope to patients facing traumatic injuries, congenital anomalies, and the aftermath of disease. In this article, we explore the latest innovations in reconstructive surgery, including tissue engineering, microsurgery, and regenerative medicine. Through a comprehensive overview of cutting-edge techniques and emerging trends, we highlight the transformative impact of these advancements on patient outcomes and quality of life. Keywords: reconstructive surgery, advancements, tissue engineering, microsurgery, regenerative medicine, patient outcomes.

Pediatric Surgical Oncology is a specialized field dedicated to the diagnosis and treatment of cancer in children. This article explores the unique challenges and advancements in pediatric oncology surgery, covering topics such as diagnosis, surgical techniques, postoperative care, and longterm outcomes. Key areas of focus include multidisciplinary collaboration, minimally invasive approaches, and personalized treatment plans. By understanding the complexities of pediatric surgical oncology, healthcare professionals can work together to improve outcomes and quality of life for young cancer patients.

Emergency General Surgery (EGS) encompasses a broad spectrum of surgical procedures performed urgently to address acute conditions such as trauma, abdominal emergencies, and infections. Despite advancements in surgical techniques and perioperative care, infectious complications remain a significant concern in EGS. These infections not only prolong hospital stays but also escalate healthcare costs and burden patients with additional morbidity and mortality. Thus, a comprehensive understanding of the factors influencing infectious complications and the implementation of evidence-based interventions are imperative to improve outcomes in emergency general surgery. This delves deeper into the nuances of infectious complications in EGS, aiming to provide insights that inform clinical practice and enhance patient care.