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Journal of Surgery

ISSN: [Jurnalul de chirurgie]
ISSN: 1584-9341

Open Access

Articles in press and Articles in process

      Research Article Pages: 1 - 7

      NVP-231, a Ceramide Kinase Inhibitor, Causes M Phase Arrest and Consequent Cell Death to Stop the Growth of Breast and Lung Cancer Cells

      Hari Prasad Sonwani*

      DOI: 10.37421/1584-9341.2024.20.126

      Context and objective: The production of ceramide-1-phosphate, which is catalysed by ceramide kinase (CerK), may influence a number of cellular processes, including inflammation and cell division. Here, we looked at how the newly created CerK inhibitor NVP-231 affected the viability and proliferation of cancer cells, as well as the function of cell cycle regulators linked to these reactions.

      Experimental methodology: Gradually increasing concentrations of NVP-231 were applied to the lung cancer cell line NCI-H358 and the breast cancer cell line MCF-7 in order to measure DNA synthesis, colony formation, and cell death. The cell cycle distribution of the cells was analyzed using flow cytometry, and alterations in the expression and activation of cell cycle regulators were found using Western blot analysis.

      Important findings: NVP-231 decreased DNA synthesis, colony formation, and cell survival in both cell lines in a concentration-dependent manner. Additionally, it caused apoptosis, as seen by elevated caspase-3 and caspase-9 cleavage and DNA fragmentation. NVP-231 reduced the proportion of cells in the S phase and caused an M phase arrest with an elevated mitotic index, as shown by increased histone H3 phosphorylation, according to cell cycle analysis. When staurosporine was added to NVP-231 therapy, the impact on the cell cycle became even more noticeable. Lastly, siRNA-induced down-regulation of CerK sensitized cells to staurosporine-induced apoptosis, although overexpression of CerK protected against this process.

      Conclusions and significance: our findings reveal for the first time the critical function that CerK plays in the regulation of the M phase in cancer cells. We propose that CerK be specifically inhibited, with the use of medications like NVP-231, in conjunction with standard pro-apoptotic treatment.

        Opinion Pages: 1 - 1

        Micronucleus Experiment in Aquaculture Industry

        Ane Galarneau

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        Mini Review Pages: 1 - 1

        Using Empty Fruit Bunch Sap as a Fire Retardants Cellulosic Substrate

        Ane Galarneau

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        Mini Review Pages: 1 - 1

        Utilizing Banana Stem Nectar as a Fire Retardants Cellulose Substrate

        Erin Persil

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        Perspective Pages: 1 - 1

        An Overview of the Expert of Cellulose Fiber Product Packaging

        Yuehe Lin

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        Brief Report Pages: 1 - 1

        A Report on Thermionic Emission

        Erin Persil

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          Review Article Pages: 1 - 7

          Comparing Lobectomy and Segmentectomy: Which Elements Affects an Optimal Oncological Outcome?

          Theoni Kontou, Michail Galanis*, Theodoros Kolokotronis and Efstratios Koletsis

          In this research work in recent years, Low Dose Computed Tomography (LDCT) is being used for lung cancer screening in high risk groups. According to USPSTF guidelines, people aged between 55 and 80 years old, who have a smoking history of 30 pack-years or have quit for less than 15 years, are recommended to take a LDCT scan. Other possible candidates for lung cancer screening can be patients with radon exposure, family history associated with lung cancer or history of pulmonary fibrosis or chronic obstructive lung disease. Detecting lung cancer at an early stage raises the question of optimal treatment and overall survival. This article aims to compare segmentectomy vs. lobectomy as surgical options, in case of stage I Non-Small-Cell Lung Carcinoma (NSCLC), ideally IA.

          In order to compare the two previously referred strategies, data has been collected from articles (40 studies have been reviewed), reviews and systematic analyses in PubMed Central and also reviewing recent literature. Tumor size and location, patient age, comorbidities and nodal status have been examined as factors that affect the choice between segmental or lobar tumor resection. R0 resection and distance between resection margin and tumor was taken into consideration.

          Segmentectomy could be an equal alternative to lobectomy in early stage NSCLC (tumor<2 cm). It could mainly be preferred for >75 y.o. and/or multimorbid patients with low cardiopulmonary reserve and struggle to survive a lobectomy. However, the anatomical segmentectomy requires among other a peripheral tumor position. Some segments, such as posterior segments of left upper lobe, are not removed separately. In this case, for anatomic reasons a bi- or tri-segmentectomy could be performed.

          As far as early stage NSCLC is concerned anatomic segmentectomy is an acceptable procedure in a selective group of patients. For a better tumor and stage classification a systematic lymph node dissection should be performed.

          Research Article Pages: 1 - 4

          Atherosclerotic Ulcer Treated with Hirudo-Therapy and Herbo-Medicinal Ointment: Marham-E-Raal

          Waseem Ahmad*, Saiyad Shah Alam, Zareena Aquil and Md Rizwanullah

          Introduction: Atherosclerosis is mainly a worsening problem related to advancing age with a chronic inflammatory involvement. It leads to the blockage of normal blood flow pathway and hence ischemia of the distal part of limb. The saliva of leech contains number of anti-thrombotic chemicals such as hirudin, bfrudin, haemadin and granulin-like. These chemicals inhibit thrombin fibrin binding and prevent formation of atheroma or plaque. Marham-e-raal is well known formulation for non-healing ulcers irrespective of their origin. It contains potent healing agents that are greatly beneficial for achieving the healing of ulcers.

          Methodology: One patient of atherosclerotic gangrene of lower limbs was taken into study after written informed consent. Hirudo medicinalis applied at the course of arteries of occlusion twice weekly and Marham-e-raal was used for local application after proper washing with Alusol (alum powder solution). The treatment continued for two months.

          Discussion: The patients especially belong to older age group and present with chronic non healing ulcer at distal limbs with severe burning pain. These ulcers are resilient to healing by modern treatment.

          Conclusion: The optimal treatment of atherosclerotic ischemic ulcer is application of medicinal leeches over the course of arteries showing occlusion with local application of Marham-e-raal.

            Editorial Pages: 1 - 2

            Edward D. Siew*

            Patients who develop post-operative Acute Kidney Injury (AKI) have a poor prognosis, especially when undergoing high-risk surgery. Therefore, the objective of this study was to evaluate the outcome of patients with AKI acquired after non-cardiac surgery and the possible risk factors for this complication. A multicenter, prospective cohort study with patients admitted to Intensive Care Units (ICUs) after non-cardiac surgery was conducted to assess whether they developed AKI. The patients who developed AKI were then compared to
            non-AKI patients.

              Research Article Pages: 1 - 3

              Madav Sri Ram

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              Catheter-Associated Urinary Tract Infection (CAUTI) accounts for 36% of the total Health-care associated infections, and are avoidable. Hence, it is important to assess the factors responsible for a catheterized patient developing UTI. A prospective cohort study was conducted in a tertiary care hospital among 196 patients from September 2014 to January
              2016 to assess the risk factors and estimate the proportion of catheterized patients developing UTI.

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Citations: 288

Journal of Surgery received 288 citations as per Google Scholar report

Journal of Surgery peer review process verified at publons

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