Simple covalent immobilization of Glucose Oxidase (GOX) on a carbon electrode surface with zero-length cross-linkers resulted in the development of a disposable glucose biosensor with Direct Electron Transfer (DET) of GOX. This glucose biosensor displayed a high electron move rate (ks,3.363 s−1) as well as great proclivity (km, 0.03 mM) for GOX while keeping intrinsic enzymatic exercises. Moreover, the DET-based glucose discovery was achieved by utilizing both square wave voltammetry and chronoamperometric procedures, and it accomplished a glucose location range from 5.4 mg/dL to 900 mg/dL, which is more extensive than most monetarily accessible glucometers. Using the negative operating potential, this low-cost DET glucose biosensor avoided interference from other common electroactive compounds and displayed remarkable selectivity. Especially for self-monitoring of blood glucose, it has great potential to monitor various stages of diabetes, from hypoglycemic to hyperglycemic states.
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