Chemical Sciences Journal

ISSN: 2150-3494

Open Access

Validated and Sensitive Spectrophotometric Method for the Determination of Amitriptyline Hydrochloride


Deepakumari HN, Prashanth MK and Revanasiddappa HD

A simple, rapid, selective and highly sensitive spectrophotometric method is described for the quantitative determination of a tricyclic antidepressant drug, amitriptyline hydrochloride (AMT) in pure and in pharmaceutical preparations. The method is based on the bromination of AMT with known excess of bromine. The unreacted bromine is determined based on its ability to bleach the dye Eiochrome blue black R quantitatively at 530 nm. Beer’s law was obeyed over the concentration range 0.0–15 µg/mL. The molar absorptivity value was found to be 1.345×104 L/moL/cm, with the corresponding Sandell’s sensitivity values of 0.0233 µg/cm2. The limits of detection and (LOD) and quantification (LOQ) are also reported for the developed method. Intra- and inter-assay precision and accuracy of the method was established according to the current ICH guidelines. Applications of the procedure to the analysis of various pharmaceutical preparations gave reproducible and accurate results. Further, No interferences were observed from excipients and the validity of the method was tested against reference method. Percent of relative recoveries values were 98.57% to 100.52%.


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